Te Arawa Swimming
Lucy SandersonBackstroke/Butterfly - (SOPHOMORE): Posted season- best times in and fly and back all in finals of events at WAC. On April TSA will send 16 swimmers to the African zonal competition in Lusaka (CANA Zone 3 & 4). In the last week of August they will. PARKSVILLE PARADE and FIREWORKS The Rotary Club of . Your Experience Includes: • Dip in the Grotto Mineral Pool • Choice of a One Hour .. Although we feel frustrated with our experience with the City of Parksville, we will continue working with the present zoning .. TOYOTA COROLLA.
As a result, our facility has adopted these practices for prolonged procedures. When planning studies that require prolonged anesthesia, strategies that prevent dermal necrosis, myopathies, and neuropathies should be weighed, in addition to monitoring standard vital parameters.
Thus, alternating pressure pads should be considered in all large animal species undergoing prolonged procedures.
On physical examination the animal was laterally recumbent with minimal response to manipulation and handling and was dehydrated.
Due to rapid clinical decline and poor prognosis, euthanasia was elected, and the animal was submitted for a postmortem examination. The animal was found to be in fair to adequate body condition score, but had generalized muscular atrophy with multifocal regions of pale tan discoloration within the skeletal muscle of the limbs and cervical region.
Diffusely, the esophagus was mottled red to tan and thickened up to 3 mm, and the spleen was massively enlarged, soft and light red. Histologic evaluation of the esophagus revealed marked circumferential and mural expansion by numerous neutrophils and macrophages with fewer admixed lymphocytes and plasma cells. The inflammatory infiltrate was primarily centered on the muscularis externa and serosa with extension into the periesophageal connective tissue and adjacent adipose, and sparing of the mucosal epithelium and lamina propria.
A similar inflammatory infiltrate was within the peripheral skeletal muscle and also within the heart surrounding vessels and within the endocardium. The splenic architecture was disrupted by florid myeloid hyperplasia and numerous megakaryocytes. These findings are consistent with idiopathic myofasciitis of ferrets, a disease of unknown etiology and pathogenesis that was first recognized in The disease typically affects young ferrets of both sexes and presents as muscle atrophy, weakness, lethargy, pyrexia, and leukocytosis of neutrophilic predominance.
Extensive evaluation for an infectious etiology has proven unsuccessful. A vaccine-related immune mediated mechanism or genetic susceptibility has also been suggested, but neither have been substantiated so the cause remains unknown. Ultimately animals affected by this disease have been unresponsive to medical interventions, and the disease has been uniformly fatal.
Fine needle aspirate was consistent with a neoplasia of epithelial origin, consisting of sheets of densely packed epithelial cells with anisokaryosis, anisocytosis, and an increased nuclear: Individual cells show abundant cytoplasm, stippled chromatin, and multiple nucleoli. Since the animal was pregnant and there were no signs of ulceration, pain, or distress, the animal was allowed to deliver and nurse the infant while being closely monitored.
Following weaning of the infant 20 weeks after initial presentation, the mother was euthanized and presented for necropsy. On gross exam, the mammary mass measured 3. Microscopically, the mass was well circumscribed with characteristics of malignancy in both stromal and glandular components. The stroma was composed of densely cellular streams and whorls of spindloid cells, with anisokaryosis, anisocytosis, and a high mitotic rate per 40X field.
Glandular structures within the mass were lined by multilayered neoplastic cells displaying multiple nucleoli, anisokaryosis, and a moderate mitotic rate. Adipose tissue infiltration was noted throughout the mass.
There was no evidence of metastasis in draining lymph nodes. In humans, biphasic malignant proliferation of cellular stromal and glandular components in the breast is uncommon and known as a phyllodes tumor. In the research setting, BAL is used to access the pulmonary mucosal surface for detection of specific cells generated by the vaccines introduced to the nonhuman primate NHP.
A BAL collection procedure consists of two parts: Typically a bronchoscope is used for intubation which allows for the technician to visually navigate past the larynx, the trachea, the bronchi, and into the lung. Once the bronchoscope intubation has been completed, the lavage of pulmonary mucosal surface is done through the bronchoscope.
When using the bronchoscope, the principle investigators too often would receive blood contaminated samples. The entire BAL procedure was reviewed after reports of the blood contaminated samples came in from the investigators. The review of the BAL procedure led to several changes to the procedure. The modified procedure consists of the following: Here, we present and illustrate the recent changes in methods to the BAL collection procedure which has effectively reduced the instances of blood contamination in the bronchioalveolar lavage samples.
The default prescription was oxytocin. After treating and tracking this condition for 2 years, we can now recommend 1 of 2 treatments, depending on breeding history. Our study included females of all backgrounds. Five treatment groups received 1 of 5 options: The best treatment of the 5 groups was found to be PGF2a cocktail.
But when the mice were grouped by breeding history, the results varied. Out of females, 96 were primiparous or first time dams.
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A total of 40 females survived, with a survival rate of Additionally, 96 were multiparous, or dams that had previous litters. A total of 47 survived, with a survival rate of PGF2a cocktail was the most beneficial in the primiparous females. Oxytocin cocktail yielded the highest resolution in multiparous females.
Regardless of the onset of dystocia, specific treatments are indicated if you have historical breeding information of your colony. While several genetically modified rodent models are available to study polycystic diseases, we report here the spontaneous occurrence of polycystic liver in CD-1 mice. Several of our 13 to month-old female sentinel mice CD-1 were found with distended abdomens, but otherwise appeared healthy.
When the mice were euthanized and necropsied, the liver was markedly enlarged with multiple fluid-filled cysts of various diameters throughout the liver.
The gross examination of the other major organs was unremarkable.
Histopathology of the liver showed multiloculated cysts with complete displacement of the hepatic parenchyma. The cystic structures were lined by cuboidal epithelium with distinct cell border and were consistent with bile duct origin. There were rare foci of minimal epithelial dysplasia with piling of epithelial cells and loss of cellular orientation, but there was no evidence of neoplastic transformation and no evidence of either cholestasis or cholangitis.
The number of hepatocytes was markedly decreased with intact portal structure. There was multifocal and mild lymphoplasmacytic, eosinophilic, neutrophilic periportal inflammation that rarely extends into the cystic spaces. The histopathological findings were consistent with primary biliary hyperplasia and polycystic liver with secondary inflammation. While single to few biliary cysts are sporadically encountered in aged mice, the spontaneous occurrence of multiple biliary cysts throughout the liver in our mice are characteristics of polycystic liver and is comparable to the polycystic liver lesions in humans.
Hence, further characterization and generating a new line of these mice with spontaneous development of polycystic liver would be a useful model to study pathogenesis and molecular mechanisms of polycystic liver. The external jugular veins, the inferior vena cava IVCand the right carotid artery were cannulated to provide sufficient ports for central line monitoring, blood draws, and administration of medication during ECC.
Surgery consisted of bilateral jugular cutdowns to isolate the right and left external jugular vessels and the right carotid artery. The right carotid artery was cannulated with a sheath catheter and extension line. All tubing was subcutaneously tunneled using a sharp tip chest tube trocar and exteriorized between the shoulders.
A midline laparotomy was performed to access and cannulate the IVC with a sheath catheter. All incisions were closed once catheter was secured and the extension exteriorized. The pigs were recovered from anesthesia and individually housed before ECC. ECC was executed by connecting circuit tubing to both jugular lines via a swivel. This surgical approach allowed successful conscious ECC of 4 pigs without complication for up to 9 hours and allows custom monitoring, sampling and intervention for dialysis, ECMO and other extracorporeal therapies.
Available scales for the assessment of sedation level have not been tested for validity or reliability.
Testing is essential to ensure acceptable agreement between raters, appropriate scale sensitivity when evaluating new sedatives and sedation protocols, and in comparing results between studies. The purpose of this study was to evaluate a sedation scale range for inter-rater reliability, construct validity discrimination between sedation levels and internal consistency relationship between scale items.
Our null hypothesis was that the chosen sedation scale would be unreliable when used by different raters and show poor discrimination between sedation protocols.DOUBLE HEAT WINNERS AT FIRST DAY OF SWIM TEAM CHAMPIONSHIP MEET - HUGE COME FROM BEHIND RELAY WIN
Sixty-two dogs scheduled to receive sedation at 2 veterinary clinics were filmed, both before and 15 minutes after receiving sedation drugs study ID: The sedation scale was composed of 7 items: Five experienced animal health technicians, untrained in use of the scale, independently scored 15 of the videos. The null hypothesis was rejected as these data show that the sedation scale can be used reliably by untrained raters and is therefore a useful tool to discriminate between different levels of sedation in dogs.
This facilitates comparison between studies employing the same sedation scale. Three days later, she had a decrease in appetite and fecal output with a 0.
Physical exam at that time was otherwise normal and ml of a commercially available recovery food was provided daily as a nutritional supplement. Sixteen days later, she had regained the 0.
A fluid-filled structure was palpated in the abdomen, but no fetuses were palpable, suggesting fetal demise due to presumptive pregnancy toxemia. Her weight had stabilized and nutritional supplementation was stopped. Over the next 5 months, mild recurrent loose stool with a few episodes of mild hematochezia were observed and treated with daily hay supplementation.
During this time, she also exhibited progressive abdominal distension. Abdominal palpation revealed a fluid consistency with no discrete masses. To determine the cause of abdominal distention, an exploratory surgery was performed, revealing numerous clear and red fluid-filled cysts 0. At one point the omentum was adhered to the colon and appeared to infiltrate the lumen.
One uterine horn was thin with a 0. Based on the extent of tissue involvement and poor prognosis, the guinea pig was euthanized. Histopathology indicated a multicystic peritoneal mesothelioma, but immunohistochemistry was required for a definitive diagnosis. Immunohistochemistry ruled out hemangiosarcoma and lymphangioma, but could not distinguish between mesothelioma and adenocarcinoma due to technical issues with staining. However, based on current findings, mesothelioma is considered the far more likely diagnosis than adenocarcinoma.
Most analgesics require repeat administration, leading to suspected animal stress secondary to handling and additional staff time requirements. Recuvyra, a topically-applied fentanyl solution, is currently approved for use in dogs. It is effective for providing up to 4 days of analgesia with one topical dose. We hypothesized that Recuvyra would be safe for use in mice, and provided multiple days of analgesia with one application to the dorsal tail base.
Mice were assessed for weight loss, behavior, and nociception for 4 days following application. Behavioral tests nest complexity, time-to-integrate-to-nest test, and open field testing were performed to assess individual mouse behavior. Nociception following application was assessed with the tail flick test.
All dosed mice initially lost between 1g and 1. Nest complexity scores decreased with dosing for all groups, returning to normal by day 4. Time-to-integrate-to-nest testing showed a decrease in nesting behavior initially. Tail-flick latencies were increased significantly for all groups at 24 hours, and gradually decreased to normal latency time by day 4 for the During open field testing, dosed mice exhibited less exploratory behavior, with lower center duration times and less rearing than control mice initially.
Defecation during open field testing was decreased on day 1 for all groups, returning to normal day 2. Passive Integrated Transponder PIT tags have been used to monitor mouse body temperature with relatively little stress on the animal.
We hypothesized that a thermal imaging camera may be used to obtain body temperatures from mice in manner that is less obtrusive than PIT tags with comparable results. PIT tags were implanted subcutaneously into 2 groups of mice. One group was then infected with Burkholderia spp, and one group remained uninfected.
Mice were assessed for clinical behavior and body temperature the day prior to infection and daily for 7 days postinfection. Clinical behavior was scored on a scale of 0- normal; 1- questionable illness; 2- mild but definitive illness; 3- moderate illness; 4- severe illness.
Body temperatures were taken with both the PIT tag, as well as thermal images of the eye, base of the ear, and flank. Although thermal imaging temperatures were lower than PIT tag temperature, they trended similarly with eye temperature being the closest to PIT temperatures followed by ear and flank.
Increased clinical behavior scores correlated well with lower body temperatures from the PIT tag and thermal imaging. This indicates that a thorough clinical observation scoring system may be a more efficient and effective to track disease progression in mice than monitoring body temperature.
A new reformulated biopolymer-based sustained-release formulation of buprenorphine Bup-SRwas recently developed with the goal of deriving a single dose, 3-day sustained analgesic effect, which would not only offer stable pain management but also decrease handling-associated stress and result in more cost-efficient pain management.
Here, the new formulation administered at 0. This plasma concentration is assumed as the minimum concentration that may result in adequate analgesia based on previous reports. Intracardiac blood samples were collected at 0, 0. Results suggested that at 1. These results indicate that a 3-day analgesic effect after subcutaneous administration of this reformulated Bup-SR is practical.
These results are promising, and suggest this Bup-SR formulation when dosed properly at 2. Ten affected guppies from 3 river drainages Caroni, Marianne, and Oropuche were sacrificed for histologic assessment. Fish from all drainages sampled were affected by multifocal trematodiasis which was often associated with chronic-active inflammatory changes. Trematodes were identified in the coelom, pericardium, liver, and peri-renal tissues of many guppies.
In 1 fish, multiple embedded trematodes were associated with development of pancreatic sarcoma. One fish from the Oropuche drainage had marked granulomatous meningitis of unknown etiology. Numerous fish also had microsporidia xenomas in skeletal muscle. Cumulatively, the findings suggest that infection with multiple infectious agents, especially visceral trematodes, was the most likely cause of morbidity and mortality in most fish. Clinical signs and mortalities were reportedly reduced in all populations following treatment.
The location of these trematodes in viscera suggests that they are of the platyhelminth group Digenea.
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Importantly, no digenean parasites have been previously described in a wild population of Trinidadian guppies. Parasite distribution in the fish populations suggest that there could be variations in parasite-host ecology across the sampled drainages. In addition, investigators should be aware that wild-caught specimens used for experimental purposes may have multiple comorbid conditions that could compromise research outcomes.
Although strict measures are taken to mitigate environmental contamination by select agents in the ABSL-3 facility, it is important to perform environmental testing in these areas periodically to ensure that the procedure and disinfection controls are working correctly.
The objective of this study was to collect and test samples in the areas housing Burkholderia pseudomallei-infected mice to determine if laboratory surfaces were being kept free of contamination. To accomplish this, a systematic grid, mixed with judgmental sampling, was used to include floor and vertical surface swabbing in the animal room, anteroom, main hallway, laboratory spaces, and exit vestibules.
For this reason, it can be used as an identifying factor for the detection of Burkholderia-specific DNA. The results from this study show that the local ABSL-3 surface environment is negative for Burkholderia contamination.
This internal quality control project confirms that the procedures and engineering standards currently in place, when properly used, control bacterial trafficking within the ABSL-3 environment. Our goal in 2 separate studies was: We did not observe writhing in any mouse euthanized by IP injection of sodium pentobarbital.
From these observations, we conclude that sodium pentobarbital induces a rapid onset of sedation based on LORR, and mice show no sign of pain following IP injection for euthanasia. Furthermore, the time to loss of righting following IP injection occurs well in advance of the time to the initial perception of pain as assessed by the paw lick test. Therefore, IP administration of sodium pentobarbital provides for humane euthanasia in mice, and acute pain due to chemical irritancy occurs after the time to loss of righting.
SP-A binds to alveolar macrophages, enhancing their ability to affix to debris and pathogens. Humans and animals deficient in SP-A are more likely to have recurrent and more severe respiratory infections.
Mice differ from humans in that they have only 1 surfactant protein, while humans have 2. Recently, humanized SFTPA1 and SFTPA2 transgenic mice used in respiratory studies, as well as knock-outs deficient in SP-A, rederived via cesarean section at our institution cultured positive for Pasteurella pneumotropica upon post-rederivation testing using contact sentinels.
Elimination of this pathogen was imperative to the outcome of the study since SP-A deficient mice would be at greater risk for respiratory infection. Previous reports show that enrofloxacin in drinking water at a daily dose of In our study, oropharyngeal cultures of colony mice revealed positive animals, and testing revealed sensitivity to enrofloxacin. All animals were treated with 0. This is the first report of successful elimination of P.
He received 10 irradiated whole blood transfusions beginning 3 days after the bone marrow transplant, 4 of which occurred in the week prior to presentation. A CBC revealed marked anemia, leukopenia, and thrombocytopenia. The monkey was diagnosed with Plasmodium species infection via examination of thick and thin blood films. Four days after diagnosis the monkey was found dead in its cage.
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Necropsy revealed severe hemolytic anemia with marked pallor, petechial hemorrhages, and scant adipose tissue stores. Significant histologic findings included erythrocytic protozoal organisms and hemozoin and hemosiderin pigments both intravascular and in multiple organs, as well as erythroid hypoplasia with myeloid hyperplasia in the bone marrow.
The Plasmodium infection was later determined to have originated from transfusions given to the monkey by two separate Indonesian-origin blood donors that tested positive for malaria by PCR. This case highlights the utility of routine screening of all blood donors for erythrocyte parasites such as Plasmodium species and Trypanosoma cruzi even when blood products are irradiated.
Cardiac arrhythmias, inconsistent end-tidal CO2 levels, and morbidity were noted, especially when isoflurane via face mask was used for induction and intubation.
We did not notice significant differences due to weight 1. Vitals signs including heart rate, respiratory rate, blood pressure, end-tidal CO2, and temperature were continuously recorded during surgery to monitor trends.
We plan to continue investigating modifications in the injectable infusion rates and isoflurane levels, which we expect will further decrease the need for inhalant anesthesia, provide additional cardiorespiratory stability, and eliminate surgical morbidity for future animals. The pig arrived 5 days previously in a group of 5. Physical exam on arrival was normal. The pig appeared to be healthy, until found at the morning health check recumbent on his left side with his neck extended, and vertical nystagmus.
He could not rise or stand. TPR was within normal limits. He would eat if hand fed, but could not raise his head. He could move his limbs and had a strong toe pinch withdrawal reflex. He had no external signs of trauma. When lifted to sternal recumbency, his head moved side to side, and had a pronounced left tilt. The results from a CBC and chemistry screen were unremarkable. Due to poor prognosis, we elected euthanasia. On gross necropsy, the left lung had focal atelectasis and the liver had mild regional scarring.
The brain appeared grossly normal. The left medial and inner ear had copious exudate. The cultured exudate grew Arcanobacterium pyogenes and Bacteriodes, both sensitive to ceftiofur. The final diagnosis was peracute otitis media and interna. Otitis is common in swine. The peracute onset of severe clinical signs of otitis interna is an unusual presentation of this disease. Physical examination revealed a large firm mass extending from the ventral neck, with respiratory distress upon restraint.
The mouse was euthanized and submitted for complete necropsy. Gross examination revealed a large, firm, lobulated, light tan mass effacing the sublingual, submandibular, and parotid salivary glands and lymph nodes. There were numerous white, soft to firm, nodules visible from the surface of the lungs. Histologic examination of salivary gland revealed an invasive, densely cellular, expansile, and infiltrative mass consisting of solid nests and acini of epithelial cells with large basophilic nuclei.
The lung parenchyma was effaced by multiple similar masses pulmonary metastasis. Separately, there was a large, focally extensive, invasive, and expansile mass composed of sheets and cords of anaplastic cells present in the midbrain. Based on these findings, final diagnoses of 1 salivary gland adenocarcinoma with pulmonary metastasis and 2 malignant anaplastic tumor of the midbrain were made. Several extra-prostatic transgene-associated neoplastic lesions have been described in TRAMP mice, including both salivary adenocarcinomas and anaplastic midbrain tumors.
The rat probasin promoter is an androgen-dependent promoter initially thought to restrict transgene expression to the prostate; however, androgen receptor expression has been described in other tissues. This is the first report of 2 different extra-prostatic transgene-associated neoplasms occurring within a single TRAMP mouse. The purpose of this report is to introduce the procedure and treatment against the infection of hookworm that occurred in our animal facility.
During the quarantine and acclimatization periods, 8 out of 12 animals showed intermittent diarrhea and adult larvae in feces. Adult parasites and fecal samples were analyzed by expert parasitologist. A direct smear was first performed followed by a zinc sulfate flotation. As a result, these were diagnosed with hookworm Ancylostoma ceylanicum which can cause diarrhea, anemia, weight loss, and hypoalbuminemia.
For treatment, Ivermectin was subcutaneously injected at a dose of 0. To confirm treatment effectiveness, microscopic examination was performed for fecal sample, which was collected 11 days after treatment.
The infection of hookworm can cause to affect the experiment results, especially for our rhesus macaques under immunosuppressive condition for xenotransplantation. For these reason, our report suggests that improved hygienic practices, regular examination, and appropriate immediate treatment against parasitic infection are required when rhesus macaques are newly acquired. That ambitious target of Trinidad and Tobago Olympic Committee president Brian Lewis is not supposed to bear fruit until From track and field I can see four, possibly five.
Right now the metres pool is deep. On form, Cedenio, the teen sensation is a candidate for a top three finish also, should he pace himself well through the rounds. So that takes care of two medals. I can also see the evergreen Cleopatra Borel collecting another top three placing in the shot put.
The older she gets the steadier her arm seems to grow. Three down, seven to go. I expect something solid from Keshorn Walcott also. His progress since his London gold strike has not been smooth. But his form this year seems to be on the improve.
His re-setting of the national record in Birmingham last month was proof positive. The Pan Am competition, devoid of the high quality Europeans should be the ideal stage for the Olympic champ to gain needed confidence ahead of the World Championships by winning another significant title.
Jehue Gordon could also get a Worlds boost with a podium finish in the hurdles which should not be beyond him at this stage. But her form since her return to competition this year certainly does not rule out a top three finish. A lot could depend on which athletes show up for these games. In the swimming pool, it would be a surprise if George Bovell leaves Toronto empty-handed.