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Primary Antibody Dilution Buffer: To Purchase S View sizes. However, STMicroelectronics assumes no responsibility for the consequences of use of such information nor for any infringement of patents or other rights of third parties which may datashedt from its use.

No license is granted by implication or otherwise under any patent or patent rights of STMicroelectronics. Western blot analysis of extracts from various cell types using Flotillin-2 L Antibody.

Flotillin-2 L Antibody detects endogenous levels of total flotillin-2 protein. It should be noted that for the best possible results a fresh datasbeet is always recommended. Find answers on our FAQs page.

L Information furnished is believed to be accurate and reliable. Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. Aspirate media from cultures; wash cells with 1X PBS; aspirate.

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Protein Blotting A general protocol for sample preparation. Electrotransfer to nitrocellulose membrane Do not aliquot the antibody. Note that the time interval frequency is always: Blotting Membrane and Paper: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.

L 데이터시트(PDF) – STMicroelectronics

Both flotillin-1 and flotillin-2 are commonly used as lipid raft-associated markers. Peak Output Current vs. Power dissipation is reduced.

Unit 4 A 0 daatasheet Changing to another country might result in loss of shopping cart. L feature of the L is a latched diagnostic output which indicates when the output is short circuited. Flotillin members are ubiquitously expressed and located in noncaveolar microdomains lipid rafts on the plasma membrane where they support signal transduction and regulate lipid raft motility and localization In the case a the main regulation loop is always closed and it forces: Flotillin-2 L Antibody – Two flotillin members have been described, flotillin-1 and flotillin Wash three times for 5 min each with 15 ml of TBST.

Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Detection of Proteins Directions for Use: Antibodies are purified by peptide affinity chromatography. Output Saturation Voltage vs.

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Dilute to 1X with dH 2 O. However, overexpressed flotillin-2 has also been found in the late endosome 4,8,9. Application Dilutions Western Blotting 1: Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry an evolutionarily conserved domain called the prohibitin homology domain PHB dataheet.

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Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. Copy your embed code and put on your site: Biotinylated Protein Ladder Detection Pack: Specifications mentioned in this publication are subject to change without notice Would you like to visit your country specific website?

Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Leu of human flotillin Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film.

Human, Mouse, Rat, Monkey.